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University of Nebraska–Lincoln

Agronomy and Horticulture

The Science and Application of Plants to Sustain and Enhance Human Life

Thomas E. Clemente

Professor

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DEGREES:

  • B.S., Biology, Indiana University of Pennsylvania, 1985
  • M.S., Plant Pathology, Oklahoma State University, 1989
  • Ph.D., Plant Pathology, North Carolina State University, 1993
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RESEARCH INTERESTS:

Employing the tools of biotechnology to broaden the fundamental knowledge of plant systems and genetically enhance crop plants with value-added and disease control traits.

COURSES TAUGHT:

  • PLPT 498/898, Plant Genetic Engineering

MAJOR PROJECT ACTIVITIES:

Developing durable field resistance towards white mold in soybean. Reduction of the polyunsaturated fatty acids in soybean seed storage lipids.

SELECTED PUBLICATIONS:

  • Zhang, Z., A. Xing, P. Staswick, T. Clemente. 1999. The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean Plant Cell, Tiss. Org Cult. 56:37-46.

  • Clemente, T., B.J. LaValle, A.R. Howe, D.C. Ward, R.J. Rozman, P.E. Hunter, D.L. Broyles, D.S. Kasten, M.A. Hinchee. 2000. Progeny analysis of glyphosate selected transgenic soybeans derived from Agrobacterium-mediated transformation. Crop Sci. 40:797-803.

  • Campbell, B.T., P.S. Baenziger, A. Mitra, S. Sato, T. Clemente. 2000. Inheritance of multiple transgenes in wheat. Crop Sci. 40:1133-1141.

  • Xing, A., S. Sato, P. Staswick, Z. Zhang, T. Clemente. 2000 The use of the two T-DNA binary system to obtain marker-free transgenic soybeans. Cell. Dev. Biol. 36:456-463

  • Dickman, M.B., Y.K. Park, T. Oltersdorf, T. Clemente, and R. French. 2001. Abrogation of disease development in plants expressing animal antiapoptotic genes. Proc. Natl. Acad. Sci. USA. 98:6957-6962.

  • Staswick, P. Z, Zhang, T. Clemente, and J. Specht. 2001. Efficient down regulation of the major vegetative storage protein genes in transgenic soybean does not compromise plant productivity. Plant Physiol. 127:1819-1826.

  •  Kinney, P. Staswick, T. Clemente. 2002. Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean. Plant J. 30:155-163.

  • Sato, S., T. Clemente, and I. Dweikat. 2003. Identification of an elite sorghum genotype with high in vitro performance capacity. In Vitro Cell. Dev. Biol.- Plant 40:57-60.

  •  Sato, S., A. Xing, X. Ye, B. Schweiger, A. Kinney, G. Graef, and T. Clemente. 2004. Production of -linolenic acid and stearidonic acid in seeds of marker-free transgenic soybean. Crop Sci. 44:646-652.

  • Kinney, A. J. and T. Clemente 2005. Modifying soybean oil for enhanced performance in biodiesel blends. Fuel Process. Technol. 86:1137-1147.

  • Howe, A., S. Sato, I. Dweikat, M. Fromm, and T. Clemente. 2006. Rapid and reproducible Agrobacterium-mediated transformation of sorghum. Plant Cell Rep. 25:784-791.

  • Vasconcelos, M., H. Eckert, V. Arahana, G. Graef, M. A. Grusak, and T. Clemente. 2006. Molecular and phenotypic characterization of transgenic soybean expressing the Arabidopsis ferric chelate reductase gene, FRO2. Planta 224:1116-1128.

  • Eckert, H., B. LaVallee, B. J. Schweiger, A. J. Kinney, E. B. Cahoon, and T. Clemente. 2006. Co-expression of the borage ∆6 desaturase and the Arabidopsis ∆15 desaturase results in high accumulation of stearidonic acid in the seeds of transgenic soybean. Planta 224:1050-1057.

  • Xu, W., S.J. Sato, T.E. Clemente, and R. Chollet. 2007. The PEP-carboxylase kinase gene family in Glycine max (GmPpcK1-4): an in-depth molecular analysis with nodulated, non-transgenic and transgenic plants. Plant J. 49:910-923.

  • Behrens, M. R., N. Mutlu, S. Chakraborty, R. Dumitru, W.-H. Jiang, B. J. LaVallee, P. L. Herman, T. E. Clemente and D. P. Weeks. 2007 Dicamba resistance: Enlarging and preserving biotechnology-based weed management strategies. Science 316:1185- 1188.

  • Fu, J., I. Momcilovic, T. E. Clemente, N. Nersesian, H. N. Trick, and Z. Ristic. 2008. Heterologous expression of a plastid EF-Tu reduces protein thermal aggregation and enhances CO2 fixation in wheat (Triticum aestivum) following heat stress. Plant Mol. Biol. 68:277-288.

  • Mathieu, M., E. K. Winters, F. Kong, J. Wan, S. Wang, H. Eckert, D. Luth, M. Paz, C. Donovan, Z. Zhang, D. Somers, K. Wang, H. Nguyen, R. C. Shoemaker, G. Stacey, and T. Clemente. 2008. Establishment of a soybean (Glycine max Merr. L.) transposon-based mutagenesis repository. Planta. 229:279-289.

  • Graef, G., B. J. LaVallee, P. Tenopir, M. Mustafa, B. J. Schweiger, A. J. Kinney, J. Van Gerpen, and T. E. Clemente. 2009. A high oleic acid and low palmitic acid soybean: Agronomic performance and evaluation as a feedstock for biodiesel. Plant Biotechnol J. 7:411-421